P-18: Effects of Ethanol Consumption on Chromatin Condensation and DNA Integrity of Epididymal Spermatozoa In Rat

Authors

  • Abbasi Sarcheshmeh A
  • Khalili MA
  • Nayeri M
Abstract:

Background: Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Both acute and chronic alcohol consumption may affect spermatozoal chromatin disorders through apoptosis. Therefore, for the first time, this experimental study was performed to evaluate the effect of ethanol consumption on sperm parameters and chromatin integrity of spermatozoa aspirated from cauda epididymis of rats. Materials and Methods: Twenty adult Wistar rats were divided into ethanol consumption and control groups. Access to ethanol and water was provided ad libitum for experimental and control animals, respectively. The cauda epididymal spermatozoa were aspirated for analysis of sperm parameters and sperm chromatin integrity with aniline blue (AB), chromomycin A3 (CMA3), toluidine blue (TB), and acridine orange (AO) assays were done. Results: Sperm progressive and nonprogressive motility of ethanol-consuming rats were significantly decreased compared with control animals (p<0.05). In addition, the rates of AB-reacted spermatozoa were similar in both groups (p<0.05). However, with regard to CMA3, AO, and TB stainings, there was a significant increase in ethanol group when compared with the controls (p<0.05). The majority of TBþ and AOþ spermatozoa were higher than ‘‘cut-off’’ value in ethanol group, whereas the mean rates of CMA3þ spermatozoa was below the ‘‘cut-off’’ value in both groups. Conclusion: The results showed that ethanol consumption disturbs sperm motility, nuclear maturity and DNA integrity of spermatozoa in rat. Therefore, ethanol abuse results in the production of spermatozoa with less condensed chromatin, and this may be one possible cause of infertility following ethanol consumption.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

full text

P-1: Morphine Has Detrimental Effects on Sperm Parameters, Chromatin Quality and DNA Integrity in Mice

Background Morphine as a natural alkaloid (opiate) is considered as one of the problems associated with poor semen production and sperm quality. For the first time, this experimental study was carried out to evaluate the impact of intra-peritoneal injection of morphine on sperm parameters, chromatin quality and DNA integrity of spermatozoa aspirated from cauda epididymis of mice. MaterialsAndMe...

full text

Effect of chromatin condensation on frozen-thawed sperm DNA integrity in normozoospermic men

Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic material of sperm is beneficial for determination of the fertility of sperm. The aim of this study was to assess the effect of sperm chromatin co...

full text

analysis of chromatin integrity and dna damage of buffalo spermatozoa

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

full text

P-134: In Vitro Assessment of Cysteine Effect on DNA Integrity of Frozen-Thawed Buffalo Spermatozoa

Background: Cysteine has been used as additive in cryopreservation extenders. It contains of thiol group which plays an antioxidant role to eliminate reactive oxygen species (ROS) during freeze -thaw procedure. Excessive ROS production cause DNA damage and subsequently reduce sperm fertility. On the other hand, protamine protein have high levels of cysteine which is critical in packaging and co...

full text

on translation of politeness strategies in dialogues involving female characters in translations and retranslations of novels translated before and after the islamic revolution of iran and their effects on the image of women: a polysystem theory approach

abstract reception environment has considerable effects on accepting a translation. as the expectations of a target culture and its values and needs change throughout history, its criteria for accepting a translation or rejecting it will change accordingly (gentzler, 2001). the expectations of iran, as the reception environment in the present study, have changed after the islamic revolution. i...

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 5  issue Supplement Issue

pages  -

publication date 2011-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023